Adrenergic Receptor Codon
By using the GCG (Genetics Computer Group, Wisconsin) map
programme
,
the codon was mapped to all known restriction endonucleases. This mapping was
done for the published sequence [77] as well as for
one including the found base substitutions (see section results). The
comparison of the two generated maps revealed that three mutations (see table
tab:alleles) altered
accompanying restriction sites: bp 1515, 1786, and 2316. As the nucleotide
exchange by mutation 1309 did not alter the
restriction maps, but was suspected to have significant influence to the
receptor, a new restriction site was introduced by generating a mismatch of the
5' oligonucleotide primer. The altered restriction sites are shown in tables
tab:1515re to tab:2316re.
Table 4.1: Restriction sites of the
wild type (above) compared to the 1515 mutant (below)
Table 4.2: Restriction sites of the
wild type (above) compared to the 1786 mutant (below)
Table 4.3: Restriction sites of the
wild type (above) compared to the 2316 mutant (below)