Stock Solutions

CaCl , 1M:
147g CaCl H O dissolved in 1 liter H O. Autoclaved, stored at room temperature.
EDTA, 0.5M:
186g/l ethylenediamine tetraacetic acid , disodium salt and 10 N NaOH to pH 8.0, autoclaved.
Glucose, 20%:
20g glucose dissolved in 100ml water, sterile filtered.
3M KAc/5M HAc:
29.4g potassium acetate and 11.5 ml acetic acid in 100 ml of water. Autoclaved.
NaAc, 3M:
408g CH COONa 3H O in 1 liter H O. Acetic acid to pH 5.2, autoclaved.
PEG, 20%:
20g Polyethylenglycol 6000  dissolved in 100ml water, sterile filtered.
SDS 20%:
200g SDS (Sodium dodecyl sulfate ) dissolved in 1 liter water at 60psy176 C, not autoclaved!
TAE buffer , 40 :
193.6g Tris-base, 15.2g EDTA-Na 2H O, 108.9g Na acetate 3H O. Acetic acid to pH 7.2, H O to make 1 liter. Stored at room temperature.
TBE buffer , 10 :
60.5g Tris-base, 30.8g boric acid and 3.72g EDTA-Na 2H O, H O to make 1 liter. Stored at room temperature. Remade if major residues appear in solution.
Gel loading buffer, 5 :
50% glycerol, 0.5% bromophenol blue , 0.5% xylene cyanol  in 5 TAE.